164.356 Metabolomics
This course is in all assigned curricula part of the STEOP.
This course is in at least 1 assigned curriculum part of the STEOP.

2022W, VO, 1.5h, 2.0EC
Lecture TubeTUWEL


  • Semester hours: 1.5
  • Credits: 2.0
  • Type: VO Lecture
  • LectureTube course
  • Format: Hybrid

Learning outcomes

After successful completion of the course, students are able to identify metabolomic questions in basic and applied research and quality control. The learning goal is to know different metabolomic strategies and choose the appropriate strategy for diverse questions. Students will be able to design different metabolomic experiments and theoretically apply sample preparation and analyses and bioinformatic analyses. This includes knowledge of metabolite extraction from biological matrices, separation techniques, such aschromatography (LC, GC, TLC), (tandem) mass spectrometry (MS/MS), ion mobility (IMS) and LC-(IMS)-MS/MS of for identification, characterisation and quantification and functional annotation of metabolites. Moreover, students will learn to apply statistical tools to metabolomic data to calculate significant quantitative changes.

Subject of course

Metabolomic strategies and methods: definition of research question, different sample preparation methods, identification, quantitation and annotation of metabolites, structural characterisation of metabolites,high-through-put technologies, (tandem) mass spectrometry, ion mobility, liquid/gas chromatography, bioinformatic tools

Teaching methods

Lecture with practical exercises (e.g. manual MS/MS spectrum interpretation, usage of webtools)

presentation and discussion of examples of metaboomic applications

Mode of examination




Course dates

Wed16:00 - 19:0005.10.2022Seminarraum BA 02B https://live.video.tuwien.ac.at/room/ba02A17/player.html
Wed16:00 - 19:0012.10.2022Seminarraum BA 02C https://tuwien.zoom.us/j/6355891383?pwd=Y1hkM3NCdGpJOWg3QkY4U2RHbXE2UT09
Wed16:00 - 19:0019.10.2022 - 16.11.2022Seminarraum BA 02A https://live.video.tuwien.ac.at/room/ba02g02/player.html
Metabolomics - Single appointments
Wed05.10.202216:00 - 19:00Seminarraum BA 02B https://live.video.tuwien.ac.at/room/ba02A17/player.html
Wed12.10.202216:00 - 19:00Seminarraum BA 02C https://tuwien.zoom.us/j/6355891383?pwd=Y1hkM3NCdGpJOWg3QkY4U2RHbXE2UT09
Wed19.10.202216:00 - 19:00Seminarraum BA 02A https://live.video.tuwien.ac.at/room/ba02g02/player.html
Wed09.11.202216:00 - 19:00Seminarraum BA 02A https://live.video.tuwien.ac.at/room/ba02g02/player.html
Wed16.11.202216:00 - 19:00Seminarraum BA 02A https://live.video.tuwien.ac.at/room/ba02g02/player.html

Examination modalities

Presentation and discussion of a metabolomics application example

Course registration

Begin End Deregistration end
21.09.2022 09:00 29.10.2022 19:00



Copies of the ppt-slides will be available via TISS.

Previous knowledge

Knowledge of biochemistry and instrumental analytical chemistry is essential. Instrumental bioanalytics is recommended.