164.235 Biomedical Mass Spectrometry
This course is in all assigned curricula part of the STEOP.
This course is in at least 1 assigned curriculum part of the STEOP.

2023S, VU, 2.0h, 3.0EC


  • Semester hours: 2.0
  • Credits: 3.0
  • Type: VU Lecture and Exercise
  • Format: Hybrid

Learning outcomes

After successful completion of the course, students are able to explain principles and mechanisms of the gaseous phase ionization techniques and desorption/ionization techniques, to identify principles and efficiencies of "high end" mass-spectrometric analyser, to categorise different mass-spectrometers as well as to name typical application examples in the field of biomedical engineering.

Subject of course

  • Principles and mechanisms of the gaseous phase ionization techniques and desorption/ionization techniques (desorption Electrospray Ionization (DESI), vacuum matrix Assisted LDI (vMALDI, ESI and nano ESI)
  • principles and efficiencies of "high end" mass-spectrometric analyzers: Linear and reflectron time-of-flight analyser, quadrupoles, 2D and 3D ion traps inclusive orbitrap
  • Tandem and multi-stage mass spectrometer for collision-induced dissociation
  • Imaging and profiling mass spectrometry
  • Application of the methods on qualitative (structure-referred) and quantitative questions from the medicine and medical pharmacology/toxicology.

Teaching methods

  • concept: inverted classroom - self study, meetings for assessment to monitor progress of study
  • practice section on mass spectrometers (at the end of the lectures)
  • slides and videos suitable to achieve the learning objective

Mode of examination




Course dates

Thu09:00 - 12:0023.03.2023 - 29.06.2023Seminarraum BA 02C Assessment
Thu09:00 - 12:0011.05.2023FH Hörsaal 3 - MATH Presentations
Thu09:00 - 12:0025.05.2023GM 3 Vortmann Hörsaal - VT Presentations
Biomedical Mass Spectrometry - Single appointments
Thu23.03.202309:00 - 12:00Seminarraum BA 02C Assessment
Thu30.03.202309:00 - 12:00Seminarraum BA 02C Assessment
Thu06.04.202309:00 - 12:00Seminarraum BA 02C Assessment
Thu13.04.202309:00 - 12:00Seminarraum BA 02C Assessment
Thu20.04.202309:00 - 12:00Seminarraum BA 02C Assessment
Thu27.04.202309:00 - 12:00Seminarraum BA 02C Assessment
Thu04.05.202309:00 - 12:00Seminarraum BA 02C Assessment
Thu11.05.202309:00 - 12:00Seminarraum BA 02C Assessment
Thu11.05.202309:00 - 12:00FH Hörsaal 3 - MATH Presentations
Thu25.05.202309:00 - 12:00Seminarraum BA 02C Assessment
Thu25.05.202309:00 - 12:00GM 3 Vortmann Hörsaal - VT Presentations
Thu01.06.202309:00 - 12:00Seminarraum BA 02C Assessment
Thu15.06.202309:00 - 12:00Seminarraum BA 02C Assessment
Thu22.06.202309:00 - 12:00Seminarraum BA 02C Assessment
Thu29.06.202309:00 - 12:00Seminarraum BA 02C Assessment

Examination modalities

  • oral examination
  • Exam Dates announced and booked via TISS

Course registration

Begin End Deregistration end
06.03.2023 12:00 06.07.2023 00:00

Registration modalities

The attendence of the laboratory course is essential to finish this VU.


Study CodeObligationSemesterPrecon.Info
066 453 Biomedical Engineering Mandatory
AM Lif e S cie nce s Austauschmodule Life Sciences Not specified


Copies of Powerpoint slides will be available via TISS.

E. de Hoffmann and V. Stroobant, Mass spectrometry - principles and applications (3rd edition) Wiley, 2007 (Paperback).

Previous knowledge

Lecture series Instrumentell Analytical Biochemistry (164.234) and basic concepts of biochemistry/chemistry.