164.235 Biomedical Mass Spectrometry
This course is in all assigned curricula part of the STEOP.
This course is in at least 1 assigned curriculum part of the STEOP.

2023S, VU, 2.0h, 3.0EC
Quinn ECTS survey


  • Semester hours: 2.0
  • Credits: 3.0
  • Type: VU Lecture and Exercise
  • Format: Hybrid

Learning outcomes

After successful completion of the course, students are able to explain principles and mechanisms of the gaseous phase ionization techniques and desorption/ionization techniques, to identify principles and efficiencies of "high end" mass-spectrometric analyser, to categorise different mass-spectrometers as well as to name typical application examples in the field of biomedical engineering.

Subject of course

  • Principles and mechanisms of the gaseous phase ionization techniques and desorption/ionization techniques (desorption Electrospray Ionization (DESI), vacuum matrix Assisted LDI (vMALDI, ESI and nano ESI)
  • principles and efficiencies of "high end" mass-spectrometric analyzers: Linear and reflectron time-of-flight analyser, quadrupoles, 2D and 3D ion traps inclusive orbitrap
  • Tandem and multi-stage mass spectrometer for collision-induced dissociation
  • Imaging and profiling mass spectrometry
  • Application of the methods on qualitative (structure-referred) and quantitative questions from the medicine and medical pharmacology/toxicology.

Teaching methods

  • concept: inverted classroom - self study, meetings for assessment to monitor progress of study
  • practice section on mass spectrometers (at the end of the lectures)
  • slides and videos suitable to achieve the learning objective

Mode of examination




Course dates

Thu10:00 - 12:0023.03.2023 - 29.06.2023Seminarraum BA 02C Assessment
Biomedical Mass Spectrometry - Single appointments
Thu23.03.202310:00 - 12:00Seminarraum BA 02C Assessment
Thu30.03.202310:00 - 12:00Seminarraum BA 02C Assessment
Thu20.04.202310:00 - 12:00Seminarraum BA 02C Assessment
Thu27.04.202310:00 - 12:00Seminarraum BA 02C Assessment
Thu04.05.202310:00 - 12:00Seminarraum BA 02C Assessment
Thu11.05.202310:00 - 12:00Seminarraum BA 02C Assessment
Thu25.05.202310:00 - 12:00Seminarraum BA 02C Assessment
Thu01.06.202310:00 - 12:00Seminarraum BA 02C Assessment
Thu15.06.202310:00 - 12:00Seminarraum BA 02C Assessment
Thu22.06.202310:00 - 12:00Seminarraum BA 02C Assessment
Thu29.06.202310:00 - 12:00Seminarraum BA 02C Assessment

Examination modalities

  • oral examination
  • Exam Dates announced and booked via TISS

Course registration

Begin End Deregistration end
06.03.2023 12:00 06.07.2023 00:00

Registration modalities

The attendence of the laboratory course is essential to finish this VU.



Copies of Powerpoint slides will be available via TISS.

E. de Hoffmann and V. Stroobant, Mass spectrometry - principles and applications (3rd edition) Wiley, 2007 (Paperback).

Previous knowledge

Lecture series Instrumentell Analytical Biochemistry (164.234) and basic concepts of biochemistry/chemistry.