After successful completion of the course, students are able to describe optical basics, explain light microscopy and fluorescence microscopy, and discuss various microscopic techniques and the concept of optical resolution. Students can describe single-molecule and super-resolution microscopy and list the latest biological applications.

• Basics in Optics: the essentials in geometrical optics and wave optics, lenses and lens aberrations
• Light microscopy: setups, resolution, image formation, contrast
• Fluorescence microscopy
• Discussion of state-of-the-art microscopy techniques: confocal microscopy, near-field microscopy, total internal reflection microscopy, ¿
• Single molecule micoscopy
• Superresolution microscopy
• New biological applications

Presentation and discussion of the subjects with the students.

Literature

A script and handouts will be offered

Oral exam on appointment. Estimated duration: 30 minutes

A script and handouts will be offered