134.232 Mammalian Cell Culture
This course is in all assigned curricula part of the STEOP.
This course is in at least 1 assigned curriculum part of the STEOP.

2022S, LU, 1.0h, 1.0EC, to be held in blocked form

Course evaluation

Properties

  • Semester hours: 1.0
  • Credits: 1.0
  • Type: LU Laboratory Exercise
  • Format: Presence

Learning outcomes

After successful completion of the course, students are able to work independently in a cell culture lab. They have mastered aseptic techniques as well as basic cell culture techniques such as cell passaging, cryopreservation and cell counting.

Subject of course

This course provides an introduction to mammalian cell culture, covering topics such as laboratory setup, aseptic techniques and safety aspects. Students will perform the following techniques on both adherent and suspension cell lines: resuspension of mammalian cells from cold storage, sterile media preparation, passaging and feeding of cells, cryopreservation of cells, microscopic evaluation of confluence and morphology, and cell counting. Further, students will work with 3D spheroid cultures and perform metabolic activity assays.

Teaching methods

The contents of the laboratory course are conveyed by a working instruction and the exercises described therein. An instructor will show and supervise small groups of students.

Mode of examination

Immanent

Additional information

Registration for the LU requires a positive completion of the accompanying VO. Modalities of the LU will be announced in the VO.

A course is scheduled for June 2022.

! Due to low capacities we can only accomodate up to 10 students !

 

If you are interested in taking the course, please contact: eva.sevcsik@tuwien.ac.at

Lecturers

Institute

Examination modalities

Immanent

Course registration

Begin End Deregistration end
31.05.2022 12:00 30.08.2022 12:00

Registration modalities

Registration for the LU will be discussed in the accompanying VO.

Precondition

The student must have at least 1 of the course(s) completed listed below:

Curricula

Literature

No lecture notes are available.

Previous knowledge

Mammalian Cell Culture, VO

Preceding courses

Language

English