After successful completion of the course, students are able to independently work in a cell culture lab as well as plan and conduct experiments with cells. They have gained theoretical knowledge of the principles and techniques in mammalian cell culture. 

• Cell culture basics (laboratory setup, equipment, aseptic technique, contamination, safety aspects)
• Commonly used cell lines (adherent and suspension cell lines), primary cell culture
• Commonly used cell culture media and supplements
• Basic cell culture techniques (cryopreservation of cells, microscopic evaluation of confluence and morphology, cell counting)
• Transfection and selection, cell line differentiation, characterization of cells
• 3D cell culture, spheroids
• Metabolic activity assays
• Bioprocessing technologies

Presentation and discussion of the subjects with the students

Depending on the circumstances, the lecture will be held on site

Lecture dates: 

 BA Seminar room 08B

13.3.2023 10:00-12:00

20.3.2023 10:00-12:00

27.3.2023 10:15-12:00

17.4.2023 10:15-12:00

BA Seminar room 10B

24.4 14:00-16:00

28.4. 14:00-16:00

Written exam

You can find more information on the manufacturer's webpages:

https://www.thermofisher.com/at/en/home/references/gibco-cell-culture-basics.html

 or

 https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/425/663/fundamental-techniques-in-cell-culture.pdf

Basic knowledge in cell biology, biochemistry strongly recommended