134.231 Mammalian Cell Culture
This course is in all assigned curricula part of the STEOP.
This course is in at least 1 assigned curriculum part of the STEOP.

2022S, VO, 1.0h, 1.5EC, to be held in blocked form

Course evaluation

Properties

  • Semester hours: 1.0
  • Credits: 1.5
  • Type: VO Lecture
  • Format: Distance Learning

Learning outcomes

After successful completion of the course, students are able to independently work in a cell culture lab as well as plan and conduct experiments with cells. They have gained theoretical knowledge of the principles and techniques in mammalian cell culture. 

Subject of course

  • Cell culture basics (laboratory setup, equipment, aseptic technique, contamination, safety aspects)
  • Commonly used cell lines (adherent and suspension cell lines), primary cell culture
  • Commonly used cell culture media and supplements
  • Basic cell culture techniques (cryopreservation of cells, microscopic evaluation of confluence and morphology, cell counting)
  • Transfection and selection, cell line differentiation, characterization of cells
  • 3D cell culture, spheroids
  • Metabolic activity assays
  • Bioprocessing technologies

Teaching methods

Presentation and discussion of the subjects with the students

Depending on the circumstances, the lecture will be held in hybrid mode

Mode of examination

Written

Additional information

Lecture dates: 

Di 26.4. 10:00-12:00

Fr 29.4. 10:00-12:00

Di 3.5. 10:00-12:00

Fr 6.5. 10:00-12:00

The Zoom link for lecture 1-6 is:

https://tuwien.zoom.us/j/97823203911?pwd=T2hSTkNIL2ptSWNnRlJldTM3VUdMdz09

Meeting ID: 978 2320 3911
Password: mb3JW5Eh

 

Mo 9.5. 13:00-15:00

Fr 13.5. 10:00-12:00

 

EXAM DATES:

May 20 and June 1st, 2022
14:00-14:30
Place: Zoom: https://tuwien.zoom.us/j/97823203911?pwd=T2hSTkNIL2ptSWNnRlJldTM3VUdMdz09

Please read the pdf describing the exam modalities in the "Unterlagen (Documents)" section.

Lecturers

Institute

Examination modalities

Written exam

 

Course registration

Begin End Deregistration end
08.03.2022 10:00 27.05.2022 23:59

Curricula

Literature

Language

German