After successful completion of the course, students are able to independently work in a cell culture lab as well as plan and conduct experiments with cells. They have gained theoretical knowledge of the principles and techniques in mammalian cell culture. 

• Cell culture basics (laboratory setup, equipment, aseptic technique, contamination, safety aspects)
• Commonly used cell lines (adherent and suspension cell lines), primary cell culture
• Commonly used cell culture media and supplements
• Basic cell culture techniques (cryopreservation of cells, microscopic evaluation of confluence and morphology, cell counting)
• Transfection and selection, cell line differentiation, characterization of cells
• 3D cell culture, spheroids
• Metabolic activity assays
• Bioprocessing technologies

Presentation and discussion of the subjects with the students

Depending on the circumstances, the lecture will be held in hybrid mode

Lecture dates: 

Di 26.4. 10:00-12:00

Fr 29.4. 10:00-12:00

Di 3.5. 10:00-12:00

Fr 6.5. 10:00-12:00

The Zoom link for lecture 1-6 is:

https://tuwien.zoom.us/j/97823203911?pwd=T2hSTkNIL2ptSWNnRlJldTM3VUdMdz09

Meeting ID: 978 2320 3911
Password: mb3JW5Eh

Mo 9.5. 13:00-15:00

Fr 13.5. 10:00-12:00

EXAM DATES:

May 20 and June 1st, 2022
14:00-14:30
Place: Zoom: https://tuwien.zoom.us/j/97823203911?pwd=T2hSTkNIL2ptSWNnRlJldTM3VUdMdz09

Please read the pdf describing the exam modalities in the "Unterlagen (Documents)" section.

Written exam

You can find more information on the manufacturer's webpages:

https://www.thermofisher.com/at/en/home/references/gibco-cell-culture-basics.html

 or

 https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/425/663/fundamental-techniques-in-cell-culture.pdf